Composite

Part:BBa_K4059040

Designed by: Julie le-Fevre Hansen   Group: iGEM21_SDU-Denmark   (2021-10-21)


PsiH from Psilocybe cubensis with N-terminal substitution (3), E. coli K12 codon optimized, with Lac

PsiH from Psilocybe cubensis with N-terminal substitution (3), E. coli K12 codon optimized, with LacI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2128
    Illegal SapI.rc site found at 1750


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Categories
Parameters
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